Next-Generation Sequencing (NGS) allows the identification of mutations in heterogeneous complex samples. But, current NGS methods are prone to errors leading to inaccurate results. Unlike true mutations, which are complementary and present on both strands, sequencing errors that occur during amplification only impact one strand.
Our proprietary error-corrected NGS (ecNGS) technology, Duplex Sequencing, virtually eliminates sequencing errors by comparing individually labeled complementary DNA strands. Our technology allows for detecting rare genetic variants with an error rate of less than 1/10,000,000 per nucleotide sequenced compared to 1/100 to 1/1,000 of standard NGS.
Example: Mutation data from our mutagenesis product.
The true variant (dark purple) can be hiding in the background of standard NGS (light purple).
The true variant easily detected with Duplex Sequencing.
TwinStrand Duplex Sequencing Kits and Services are For Research Use Only. Not For Use in Diagnostic Procedures.