Duplex Sequencing studies provide the Mutation Frequency (MF) data you need for mutagenicity testing and BMD modeling without having to wait for a TGR study.
TwinStrand now offers mutagenicity studies for nitrosamine impurities both in-house and through select service providers.
Duplex Sequencing has been successfully applied to nitrosamine testing. The Bercu et al. paper provides an excellent template for nitrosamine studies using ecNGS. The HESI position paper offers expert perspective on applications for ecNGS in nonclinical genotoxicity testing. More publications featuring nitrosamine and bridging studies can be found on the publications page.